Course Materials Needed: Where to Find Supplies?
To actively complete this class, you will need these supplies
| "Household" Items | Source | X |
| microwave oven (with a turntable) or a pressure cooker | kitchen, grandmother, discount store | |
| 40+ baby food jars (100 = optimum) | daycare centers, neighbors, friends, garage sales | |
| 2 pint jars (regular opening) | canning supplies, neighbors, friends, garage sales | |
| 1 quart jar | canning supplies, neighbors, friends, garage sales | |
| 1 tablespoon measuring spoon | kitchen | |
| table sugar (granulated sugar or cane sugar; sucrose) | kitchen | |
| vinegar | kitchen | |
| baking soda | kitchen | |
| food coloring (optional) | kitchen | |
| bleach | kitchen | |
| dish detergent, liquid (not dishwasher detergent) | kitchen | |
| isopropyl alcohol or ethanol (70%) | discount stores such as Wal-Mart; pharmacy | |
| hydroponic fertilizer (such as Peter's 20-20-20 or MiracleGro) | grocery store, garden store | |
| florist's tape | Wal-Mart, Kmart, crafts stores | |
| salad plate for cutting sterile plant material | kitchen, yard sales | |
| kitchen knife (about 6 inches) | kitchen | |
| Pyrex pie pan (about 8 inches) to hold baby food jars with media while in the microwave | kitchen | |
| protective clothing: goggles, gloves, dust mask, apron, shoes | home; Wal-Mart; hardware store | |
| plastic or cardboard box or PVC pipe and fittings to serve as a "clean area" | discount stores such as Wal-Mart; Staples, Lowes, Home Depot | |
| forceps (8 inch stainless steel is preferred) | fishing supplies store veterinary supplies Carolina Biological - #624322 - about $7.00 PhytoTechnology Labs - #F951 - about $7.50 |
|
| White Plastic jar caps for regular pint jars | WalMart grocery stores |
|
| Media solidifiers (gelatin, starch, Sure Gell, agar flakes, sand) | grocery store health food store |
|
| Test tube (25 x 150 mm) or tall shot glass (for holding alcohol that is used to soak instruments) | yard sales Wal-Mart/KMart |
|
"Scientific" Items |
Source |
|
| pH paper (wide range and narrow range) | pet stores, pool stores Nasco: #SB08261M Wide-Range pH Paper #SB20189M Dip & Read Rain Survey Kit - pH 3-6 Kitchen
Culture Kits, Inc. (see site) |
|
| handheld pH meter (an option to using pH paper; more costly but easier to use) | Nasco - www.enasco.com/prod/Home #SB22540M (about $30 plus S&H) | |
| Plant tissue culture medium
MS Basal with vitamins (6 packets = minimum) |
Kitchen Culture Kits, Inc. (go to
site for price) PhytoTechnology Labs Caisson Labs: Carolina Biological: |
|
| Plant hormones BAP (5 ml minimum) |
Kitchen Culture Kits, Inc. (go to
site for details) PhytoTechnology
Labs |
|
| Agar (need about 30 grams) | Carolina: WW-19-8206 TC Agar 8-g $5.25 4 bottles = $21.00 PhytoTechnology Labs: A111-100G Agar$15.50 Kitchen Culture Kits, Inc. (go to site details) |
|
| 100 B-caps for baby food jars (can
be microwaved and pressure cooked) ONLY NEEDED IF YOU DO NOT HAVE A PRESSURE COOKER |
Kitchen Culture Kits, Inc. PhytoTechnology Labs - C903-100EA $32.50+ Carolina Biological - WW-19-9064 $39.70 with free shipping |
|
| PPM = Plant Preservative Mixture
(a biocide that limits contamination in cultures) 10 ml minimum |
PhytoTechnology Labs Plant Cell Technology Kitchen Culture Kits, Inc. |
Best sources for difficult to find items
|
Carolina Biological, www.carolina.com
|
Caisson Laboratories, www.caissonlabs.com (media, chemicals, etc.)
|
Nasco - www.enasco.com/prod/Home
|
Phytotechnology Labs - www.phytotechlab.com
|
| Kitchen Culture Kit - manual, supplies, media, kit, CD, workshops; offers super packages for online students: http://www.kitchenculturekit.com/catalog.htm |
Kitchen Culture Kits, Inc. Super Package
Offers for Online Class
(Order online at www.kitchenculturekit.com/catalog.htm
)
| Item | Minimum | Minimum Plus 1 |
Minimum Plus 2 |
Special Package Expanded for Online Class |
| MS Basal Medium with Vitamins | 5 | 5 | 6 | 6 |
| Knudson C Medium for Orchid Seed Culture | - | - | 1 | 1 |
| Hormones (5 ml each at 1 mg/ml): | ||||
| 24D | 1 | 1 | 1 | 1 |
| IAA | 1 | 1 | ||
| IBA | 1 | 1 | ||
| NAA | 1 | 1 | ||
| BAP | 1 | 1 | 1 | 1 |
| Kinetin | 1 | 1 | ||
| 2iP | 1 | 1 | ||
| Agar (9 gm) | 6 | 6 | 6 | 6 |
| 1 ml transfer pipettes | 4 | 4 | 8 | 8 |
| Peter's fertilizer (1 tsp) | 2 | 4 | 4 | |
| pH papers | 1 | 1 | 1 | |
| CD with MSDS info | 1 | 1 | 1 | 1 |
| Plant Tissue Culture Interactive CD | 1 | |||
| Pink spoons for measuring agar | 1 | 1 | 1 | 1 |
| B-caps for baby food jars (120) | 1 | 1 | 1 | |
| Pint jar covers, plastic, 2 (for microwaving water) | 1 | |||
| PPM (5 ml) | 1 | |||
| Measuring spoons | 1 | |||
| Syringe, 3 ml | 1 | |||
| Vinyl gloves (pair) | 1 | |||
| Kitchen Culture Kit Manual | 1 | |||
| Forceps, 8 in | 1 | |||
| Cost without shipping and handling | $30 | $80 | $98 | $180 |
From: www.kitchenculturekit.com
Questions? Contact kck@turbonet.com or cstiff@austincc.edu