No changes to the lab procedure.
There will be no lab report due. Yu are expected to know and understand what you did.
Real Data:
The following figure was recently published in the scientific journal Bioscience, Biotechnology and Biochemistry. It shows the elution of a cysteine protease found in lysozymes. This figure is similar in scope to the one you should be sketching from the data on page 8a-5 in the prelab to this lab. In the figure, there are two plots: one is the A280 of each fracton which indicates the presence of protein (any protein) indicated by the open circle plot and the other indicates the activity of an enzyme (each fraction collected was assayed for cysteine protease activity and that is what is plotted below using filled squares). The brown line labeled 2 points to a protein peak (A280) at approximately fraction 75; the blue line labeled 1 points to an enzyme activity peak also at fraction 75. This indicates that the protein in fraction 75 is the enzyme that they are looking for. Note, when you do the column chromatography, you will collect about 15 fractions - this experiment collected 100 fractions!