Panel Case Studies
Correct interpretation of panel studies requires PRACTICE. The following case
studies will illustrate a systematic method of evaluating and determining the
specificity of the antibody present. REMEMBER, antigens are present on the RED
BLOOD CELLS. A positive reaction with a cell indicates the presence of an ANTIBODY
in the patient sample directed against an ANTIGEN present on the reagent cells.
Careful evaluation of the positive and negative reactions will frequently indicate
the specificity of the antibody.
An excellent overview of the process is illustrated in a PowerPoint presentation
created by Renee Wilkins, PhD, MLS(ASCP)cm from the University of Mississippi
Medical Center: Antibody Identification
Crucial to the investigation of the antibody is the patient's history:
- Has the patient ever been pregnant? Pregnancy can cause immunization of
the patient to an antigen on the fetus cells that the mother LACKS and which
was inherited from the father.
- Has the patient ever been transfused? Transfusion exposes the patient to
RBC antigens they do not possess and can cause immunization to antigens they
lack but are present on the donor RBCs.
- What is the patient's diagnosis? This may offer clues as to the patient's
exposure to RBC antigens. Perhaps the patient has sickle cell anemia, a frequently
transfused population, perhaps the patient is pregnant or the diagnosis may
be of little use as it may be something like "weakness".
- What medications is the patient taking, here in the hospital AND at home?
This information is only useful if the autocontrol and DAT on the patient
- Print out the panel for each case study. Highlight
the panel, click on "file", "print" and click the radio
- Review the patient history.
- FOLLOW THE INSTRUCTIONS CAREFULLY for evaluating the panel.
- Write down any questions you may have for your professor.
- Evaluate the practice panels. If you CANNOT correctly identify the specificity
of each antibody meet with your professor for additional assistance.
Carefully review the tutorial for selecting positive
and negative controls for testing reagent anti-serums in the blood bank:
Last Update: March
Web Author: Terry Kotrla, MS, MT(ASCP)BB
Copyright ©2011 by Terry Kotrla - All Rights Reserved