- Define "unexpected" and "clinically significant" alloantibodies.
- Describe how sensitization to red blood cell antigens occur.
- Describe the importance of characterizing the clinical significance of antibodies encountered.
- Describe in detail the protocol which must be followed in working up a patient with an unexpected alloantibody.
- List the serologic characteristics which will aid in the identification of unexpected antibodies.
- Discuss the special considerations of certain Rh antibodies.
- State the importance of phenotyping the patient for the antigen to which their antibody is directed.
- State the minimum number of cells which must be positive and negative for the antigen to which the antibody being investigated and the limitations which may be encountered.
- Describe the difficulty in working up multiple antibodies and some techniques utilized in troubleshooting them.
- State the best source of donor blood for a patient who has an antibody to a high incidence antigen.
- State why transfusion should not be delayed when dealing with an antibody to a low incidence antigens.
- Describe two possible causes of anomalous serological reactions when performing compatibility testing.
- List six procedures utilized to increase and/or enhance reactivity of weakly reactive antibodies.
- Name the 2 thiol reagents used in the blood bank and describe how they work.
- State the purpose of performing a pre-warmed technique and state how this procedure is performed.
- List three types of inhibition tests routinely used in the clinical blood bank and the sources of the reagents.
- State the purpose of performing antibody titers and list three situation in which titration studies are useful.
- Describe the purpose of the adsorption procedure and list 5 applications in blood bank testing.
- Describe the elution procedure including: definition, applications and factors which influence the success of the procedure.
Last Update:
September 20, 2009
Web Author: Terry Kotrla, MS, MT(ASCP)BB
Comments: kotrla@austincc.edu
Copyright ©2000 by Terry Kotrla - All Rights Reserved - Author's
permission required for use of materials