MLAB 2431 Immunohematology
 

Unit 7 Objectives: Rh Blood Group System

  1. List the five alleles (antigens) of the Rh blood group system.
  2. State the names of the individuals responsible for identifying the Rh system.
  3. Explain how the term "Rh" factor first came to be used.
  4. Describe the clinical significance of the D antigen.
  5. Define "immunogenicity".
  6. Describe and compare the Fisher-Race, Wiener, Rosenfield and Tippett theories of inheritance of the Rh system.
  7. List the 8 Rh phenotypes in both Fisher-Race and Wiener.
  8. State the most common Rh positive and Rh negative genotypes in both Fisher-Race and Wiener.
  9. Describe the nomenclature used by the International Society for Blood Transfusion (ISBT)
  10. Given a phenotype, determine the most probable Rh genotype in both Fisher-Race and Wiener shorthand.
  11. Describe why it is important to know the race of an individual when determining an Rh genotype.
  12. Describe the three different ways that the weak D phenotype occurs.
  13. Explain the significance of weak D individuals blood donors.
  14. Explain the significance of weak D individuals as blood recipients.
  15. Define "compound antigen" and give one example found in the Rh system.
  16. Describe the “f” and “G” antigens and the antibodies produced.
  17. Describe the D deletion genotypes and the problems these genotypes may cause.
  18. Describe the phenotype and problems caused in individuals of the Rh null phenotype.
  19. Describe the red blood cell morphology of an individual with the Rh null phenotype.
  20. Describe the LW system including antigen and antibodies involved.
  21. Give an example of a variant Rh antigen.
  22. State the clinical significance of Rh antibodies.
  23. List the in-vitro characteristics of Rh antibodies.
  24. List the Rh antigens in the order of immunogenicity from most to least immunogenic.
  25. State the type of hemolysis which occurs with Rh antibodies.
  26. State the most commonly encountered Rh antibodies.
  27. State why anti-D may react more strongly with R2R2 than R1R1 red blood cells.
  28. Define "concomitant antibodies" and give one example that is of critical importance in transfusion medicine.
  29. Describe the 4 types of antisera available for D typing including: preparation, use, advantages and limitations of each.
  30. Describe the control seras used in the D typing test and when they are required.
  31. State three precautions which must be considered when using Rh typing sera.
  32. State the quality control which must be performed when using Rh typing sera.
  33. List 5 causes of false positives and 8 causes of false negatives when performing Rh antigen typing.
  34. Given the reactions of cells tested with the 5 Rh typing sera state the phenotype with 95% accuracy.
  35. Given the Rh phenotype determine the most probable Rh genotype in both Fisher-Race and Wiener shorthand with 95% accuracy.
  36. Given a genotype in either Fisher-Race or Wiener shorthand convert to the other nomenclature with 95% accuracy.
Last Update: February 12, 2011
Web Author: Terry Kotrla, MS, MT(ASCP)BB
Comments: kotrla@austincc.edu
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