Molecular Diagnostics

Austin Community College
Molecular Diagnostics Certificate Program

Information Session Presentation powerpoint

• Handout
• Information Session Attendance Form

Text and Reference Books

• Molecular Diagnostics: Fundamentals, Methods and Clinical Applications, Lela Buckingham and Maribeth Flaws, Copyright 2007, ISBN-13: 978-0-8036-1659-2, ISBN-10: 0-8036-1659-7
  ISBN-10: 0-8036-1659-7
• Fundamentals of Molecular Diagnostics, David E. Bruns, Edward R. Ashwood, and Carl A. Burtis, Copyight 2007, ISBN-10: 1416037373
• Molecular Diagnostics, George Patrinos and Wilhelm Ansorge, Elsevier Academic Press, Copyright 2005, ISBN 0-12-54-6661-7
• Molecular Diagnostics: For the Clinical Laboratorian, William B. Coleman and Gregory J Tsongalis, Humana Press, Copyright 2006, ISBN 1-58829-356-4
• Molecular Microbiology: Diagnostic Principles and Practice, David Persing, Fred Tenover, Copyright 2003, ISBN-13: 9781555812218, ISBN: 155581221X
• Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 4th Edition , Carl A. Burtis, PhD, Edward R. Ashwood, MD and David E. Bruns, MD, Copyright 2006, ISBN: 0721601898, ISBN-13: 9780721601892
  

Back to MLAB 2321 Molecular Diagnostics for Clinical Laboratory Science

NOTE: Some links below are listed twice in different areas to more easily find specific information

Remember: Right click on the link to open in a new window.

Agencies and Organizations

• Center for Biologic Evaluation and Research (CBER)
• CLIA Regulations
• Clinical and Laboratory Standards Institute (formerly NCCLS)
• College of American Pathologists (CAP) - this organization has a FREE publication which is EXCELLENT. Go to the CAP Today website for more information.
• DNA Advisory Board Quality Assurance Standards for Forensic DNA Testing Laboratories
• National Office of Public Health Genomics - (CDC)
• Nuclear Regulatory Commission
• Scientific Working Group on DNA Analysis Methods
• The Secretary's Advisory Committee on Genetic Testing has a draft report U.S. System of Oversight of Genetic Testing:
  A Response to the Charge of the Secretary of HHS , which is 192 pages long, addressing policy issues raised by the development
  and use of genetic technologies and their integration into clinical and public health practice. The home page has the bullets which the report addresses in detail. This report is open for comment until December 21, 2007. This demonstrates the steps being taken to address the moral and ethical issues of genetic testing.
• US Department of Tranportation - Pipeline and Hazardous Materials Safety Administration

Articles

• Lab-on-a-Chip Integration Theme: The Great Challenge
• Beckman-Coulter
• National Cancer Institute Biometric Research Branch - very nice journal articles

Blood Bank

• Approximately half of the erythroblasts in maternal blood are of fetal origin
• Molecular Biology of partial D and weak D: Implications for Blood Bank Practice
• Molecular Genetic Blood Group Typing by the Use of PCR SSP Technique
• Obstetric outcome after RhD and Kell testing
• Overview of Molecular Methods in Immunohematology

Breast Cancer

• Genomics and Molecular Taxonomy of Breast Cancer: Identification of New Prognostic and/or Predicative Factors

Certification

• ASCP Technologist in Molecular Pathology Examination Content Guideline
• ASCP Technologist in Molecular Pathology - Suggested Reading List For Examination Preparation

Chromosome Analysis

• Chromonsome Map of Genetic Disorders
• Fish Mapped BACs - Select a chromosome to view the chromosome's reference ideogram and an ordered list of FISH-mapped BACs, with links to CGAP and NCBI related databases. Fascinating! - The Cancer Genome Anatomy Project
• The Cancer Genome Anatomy Project - homepage
• The Cancer Genome Anatomy Project - Educational resources - very nice tutorials

Cystic Fibrosis

• Biochip Based Cystic Fibrosis Carrier Screening - Osmetech Molecular Diagnostics

Databases

• Cystic Fibrosis Mutation Database
• Human Mitochondrial Genome

DNA

• Biology Page has a number of excellent resources illustrating DNA Replication, Sequencing, and Mutation.
• DNA and the Genome - The OpenLearn website gives free access to course materials from The Open University. The LearningSpace is open to learners anywhere in the world.
• DNA Tutorial - 101science.com
• Fingerprinting Methods for Identification of Variable DNA in Genomes
• Replication Of DNA How is cellular DNA copied?
• Review the animations at the McGraw-Hill Biology site
• Virtual Laboratory: DNA Extraction

Education

• Gen Probe Posters
• Molecular Diagnostic Science Program, The Division of Clinical Laboratory Science , The University of North Carolina at Chapel Hill, Establishing a new clinical site
• Webinars - Qiagen

Enzyme Linked Immuosorbent Assay (ELISA)

• ELISA animation
• ELISA animation
• ELISA animation - prengancy test
• ELISA animation comparing direct and indicrect ELISA
• ELISA Biocrawler

DNA Library

• cDNA Library - Wikipedia
• Molecular Cloning
• Genomic and cDNA Libraries

Fluorescent In-Situ Hybridization (FISH)

• An Electronic Textbook on Basic Fluorescent In-Situ Hibrization
• Assessing residual leukemia through fluorescence in situ hybridization (FISH)
• Cytogenetics with special reference to domestic animals - although animals are the primary focus this has very nice basic explanations of FISH
• Fluorescent In Situ Hybridization: (FISH) Created by George Rice, Montana State University
• Fluorescence in Situ Hybridization (FISH) - Science Encyclopedia
• Missing genes - description and pictures of some genetic abnormalities
• What Is FISH - Abbott Laboratories

Forensics

• Iowa Division of Criminal Investigation
• FBI Publications
• Forensic Applications of Genetics & DNA
• Forensics Biotechnology
• Quiagen
• Scientific Working Group on DNA Analysis Methods (SWGDAM)
• Selected Forensic Links

Gel Electrophoresis

• DNA Gel Electrophoresis
• Gel Electrophoresis of DNA
• Gel Electrophoresis Animation
• Gel Electrophoresis - Animated tutorial Cold Harbor
• Gel Electrophoresis - Proteomics
• Introduction to Recombinant DNA technology Restriction enzymes and gel electrophoresis - restriction endonucleases very nicely done
• Top 10 Fun Facts for DNA Electrophoresis
• Virtual Laboratory: Gel Electrophoresis

Genetics

• An Animated Primer on the Basics of Genes, DNA and Heredity
• DNA Interactive
• Genetic Origins
• Genetics Science Learning Center
• Harry Potter Genetics - powerpoint
• Human Genetics - nice section on ethics, Dave Ussery, Roanoke College
• Human Genetics for M-1 Students -University of Illinois at Chicago, College of Medicine, Department of Molecular Genetics
• Human Pedigree Analysis - autosomal recessive and dominant examples
• Human Pedigree Analysis 1
• Learn Genetics
• Mendelian Inheritance - X-Linked Inheritance
• My DNAi
• Pedigree Analyis - powerpoint
• Pedigree: Working Out Inheritance Patterns - powerpoint
• Real-world Genetics

Genomics

• Primer on Medical Genomics Part II: Background Principles and Methods in Molecular Genetics - Mayo Clinic

Glossary

• A Glossary Of Terms Commonly Used In Molecular Biology
• Glossaries of Genome / Human Genetics Terms - list of glossary sites
• Glossary of Terms Used in Molecular Genetics
• A Molecular Biology Glossary - A Quick and Dirty Reference to Terms Used in Molecular Biology
• DNA Heritage
• Gene Links - University of Washington
• Glossary of Biochemistry and Molecular Biology
• Glossary of Molecular Biology Terminology - scroll down page to see glossary

Human Papilloma Virus

• Reproducibility of HPV DNA Testing by Hybrid Capture 2 in a Screening Setting - Nov 2005

Just For Fun

• Cloning Man's Best Friend: Dog DNA - miss your best friend? Find answers here!

Hybridization

• Hybridisation - includes microarrays, comparative genomic hybridisation and FISH
• Hybridization: Theory and Practical Aspects
• Nucleic Acid Hybridizations

Meiosis and Mitosis

• Meiosis - Animated Tutorial
• The Cell Cycle & Mitosis Tutorial
• Overview of Meiosis

Microarray

• Assay: Protein Microarrays on Nitrocellulose SlidesAssay: Protein Microarrays on Nitrocellulose Slides
• Demystified … Tissue microarray technology
• DNA Chips: A Laboratory In The Palm Of Your Hand
• DNA Microarray - animated tutorial
• Introduction to DNA microarray technologies
• Introduction to Microarray Technology
• Macroarray versus Microarray - end of presentation (about slide 75) more basic
• Microarray animation
• Microarray - list of links with tutorials and animations
• Microarray Tutorial
• Microarray Technologies ppt
• Preparation of DNA Microarray Biochips for the Classroom
• Tissue Microarrays: A Current Medical Research Tool
• Virtual Laboratory: DNA Microarray

Mitochondrial DNA

• Defense Prisoners of War/Missing Personnel Office
• Mitochondria and Mitochondrial DNA

Newborn Screening

• Development of Matched Phenotypic and Genotypic Control Materials in Dried Blood Spots

Newsletters/ News Sites

• Lab On A Chip

Oncology

• Assessing residual leukemia through fluorescence in situ hybridization (FISH)
• Genetic alteration and gene expression modulation during cancer progression - 2004

Paternity Testing - Most of these are commercial sites, but still interesting

• Paternity and Family Relationship Testing - DNA Diagnostic Center
• Genetic Identity
• Understanding your Paternity Test Results - Reliagene
• Worldwide Genetics - amazing how cheap the testing is now!

Pedigrees

• How to Draw Your Pedigree
• Human Pedigree Analysis
• Human Pedigree Genetics Problem Set
• PediDraw: A web-based tool for drawing a pedigree in genetic counseling
• Human Pedigree Analysis 1

Pharmacogenetics

• Roche
• Streamlining Pharmacogenetic Analysis - A person’s genetic make up can have a profound influence on how he or she will respond to a drug

Polymerase Chain Reaction (PCR)

• PCR Animation Links
• PCR for Dummies
• PCR Tutorial for Absolute Beginners
• Protocol for PCR with Taq DNA Polymerase
• The World Famous PCR Jump Station: Polymerase Chain Reaction
• Scorpions Reaction Scorpions™ is a homogeneous or closed-tube platform for PCR analysis.

PowerPoint Presentations

• Biochemistry Basics - list of powerpoint presentationsPaternity Test Glossary
• Molecular Diagnosis of Bacterial Infections: Current Status and Future Trends
• Use of Molecular Methods for Diagnostics of Infectious Diseases

Primer Design

• Primer Design - short tutorial which includes the 7 basic rules for primer design.
• PCR Primer Design Guidelines Premier Biosoft
• Integrated DNA Technologies - Wonderful site to create your own primers.

Proteomics

• Proteomics Technologies

Restriction Fragment Length Polymorphisms (RFLPs)

• Self Quiz Ch.20: DNA Technology - Quiz
• Recovery of DNA from Aged Bloodstains
• RFLP animation
• RFLP Wikipedia
• RFLP Computationaal Biology - Nice overview of the procedure.
• Restriction Fragment Length Polymorphisms (RFLPs)
• The Case of the Crown Jewels: Investigate a Crime Scene Using DNA Restriction Analysis (DNA Fingerprinting)

RNA

• Anti-Sense RNA
• DNA, RNA, and Protein Synthesis - be sure to take the quiz for each unit
• Genetic Code - explanation of sense and anti-sense strand
• Introns - Widipedia
• Introns and Exons
• Nucleic acid structure and function - nice descriptions of the different types of RNA
• Polyadenylation - Wikipedia
• RNA - Wikipedia
• Template versus Coding Strand
• The Functions of Introns: From Junk DNA to Designed DNA
• Transcription Tutorial
• Transcription Animation

Single Nucleotide Polymorphisms (SNP)

• EMBL
• International HapMap Project
• SNP Fact Sheet
• Single Nucleotide Polymorphism - NCBI

Solution Hybridization

• New Tools for miRNA and siRNA Analysis
• Isolation and Detection of Small RNA Molecules - powerpoint Ambion

Short Tandem Repeats (STR)

• Short Tandem Repeat DNA Internet DataBase

Temperature of Melting

• Comparison of different melting temperature calculation methods for short DNA sequences
• Description of the methods for DNA/DNA melting temperature calculations
• DNA Melting - Wikipedia
• HYBRIDIZATION NOTES - Melting and Annealing Temperature - Dr. Bart Frank, Arthritis and Immunology Program, OMRF
• Melting Temperature of Oligonucleotides - Sigma Aldrich
• Online Melting Temperature (Tm) Calculation
• Thermodynamics of DNA Duplex Denaturing - MSU

Testing - Sites with Lists of Testing

• A Science Primer - NCBI
• Biochem4schools
• Case Studies in Cell and Molecular Biology for post-16 Students
• Clinical Molecular Diagnostic Laboratory -
• Department of Laboratory Medicine, Molecular Diagnostics, Yale University
• Molecular Diagnostics - University of Pittsburg Medical Center
• Molecular Diagnostics Laboratories - Cincinnati, Ohio
• Molecular Diagnostics Laboratory - MD Anderson

Transplants

• Detection of Donor-specific DNA Polymorphisms in the Urine of Renal Transplant Recipients

Tuberculosis

• Automated DNA Fingerprinting Analysis of Mycobacterium tuberculosis Using Fluorescent Detection of PCR Products
• Molecular Epidemiology of Mycobacterium tuberculosis Strains Isolated from Different Regions of Italy and Pakistan
• Rapid Discrimination of Mycobacterium tuberculosis Complex Strains by Ligation-Mediated PCR Fingerprint Analysis

Tutorials

• Bio-Body Guard
• BIOL 114 Genetics and Development
• Biology I Animations Movies & Interactive Tutorial Links
• DNA Anatomy
• DNA Microarray Flash Tutorial
• Education Resources — Graphics, Animations, Tutorials, and More - BioRad
• ELISA animation
• ELISA animation
• ELISA animation - prengancy test
• ELISA animation comparing direct and indicrect ELISA
• Gel Electrophoresis Animation
• Gen Probe - Earn FREE CEUs
• Genome Analysis: Microsatellites or SNPs
• Get a Clue
• Introduction to DNA microarray technologies
• John Hopkins Presentations
• John Hopkins Molecular Pathology Diagnostics Laboratories Assay Tutorials
• Microsatellite DNA Methodology
• Microarray Technologies ppt
• Microarray Tutorial
• Microarray animation
• Molecular Diagnostics Web Links - University of North Carolina at Chapel Hill
• Molecular Searching Techniques - overview of Western, Southern, and Northern blots, FISH, RFLP
• PCR Animation Links
• Quiagen Webinars - FREE
• RFLP animation
• Scorpions Reaction Scorpions™ is a homogeneous or closed-tube platform for PCR analysis.
• Understanding Cancer Series: Molecular Diagnostics - National Cancer Institute

Western Blot

• Western Blot -
• Southern, Northern, Western, and South-Western Blots

Y-STR

• Introducing Y-RSTR in Court - pdf article
• Strengths and weaknesses associated with Y-STR genotyping
• Y-STR - DNA Diagnostics Center
• Y-STR - Wikipedia
• YHRD - Y Chromosome Haplotype Reference Database

Sites with Educational Links

• Biofluorimetry Lab
• Biology - Alief ISD, Houston, TX
• Cytogenetic Resources
• DNA forensic and genetics links
• Genomic Medicine - open source courseware from MIT, audio lectures
• Graphics Gallery - Looking for graphics? The Graphics Gallery is a series of labeled diagrams with explanations representing the important processes of biotechnology.
• Introduction to Human Genetics 2005 - Jonathan Wolfe, London's Global University
• National Human Genome Research Institute
• Quiagen Webinars - FREE
• www.101science.com

Last Update: November 14, 2012
Web Author: Terry Kotrla MT(ASCP)BB
Comments: kotrla@austincc.edu
Copyright ©2007 by Terry Kotrla - All Rights Reserved