Text and Reference Books

• Molecular Diagnostics: Fundamentals, Methods and Clinical Applications, Lela Buckingham and Maribeth Flaws, Copyright 2007, ISBN-13: 978-0-8036-1659-2, ISBN-10: 0-8036-1659-7
  ISBN-10: 0-8036-1659-7
• Fundamentals of Molecular Diagnostics, David E. Bruns, Edward R. Ashwood, and Carl A. Burtis, Copyight 2007, ISBN-10: 1416037373
• Molecular Diagnostics, George Patrinos and Wilhelm Ansorge, Elsevier Academic Press, Copyright 2005, ISBN 0-12-54-6661-7
• Molecular Diagnostics: For the Clinical Laboratorian, William B. Coleman and Gregory J Tsongalis, Humana Press, Copyright 2006, ISBN 1-58829-356-4
• Molecular Microbiology: Diagnostic Principles and Practice, David Persing, Fred Tenover, Copyright 2003, ISBN-13: 9781555812218, ISBN: 155581221X
• Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 4th Edition , Carl A. Burtis, PhD, Edward R. Ashwood, MD and David E. Bruns, MD, Copyright 2006, ISBN: 0721601898, ISBN-13: 9780721601892
  

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Back to MLAB 2378 Fundamentals of Molecular Diagnostics
Back to MLAB 2479 Molecular Diagnostics Techniques

NOTE: Some links below are listed twice in different areas to more easily find specific information

Remember: Right click on the link to open in a new window.

Agencies and Organizations

• Center for Biologic Evaluation and Research (CBER)
• CLIA Regulations
• Clinical and Laboratory Standards Institute (formerly NCCLS)
• College of American Pathologists (CAP) - this organization has a FREE publication which is EXCELLENT. Go to the CAP Today website for more information.
• DNA Advisory Board Quality Assurance Standards for Forensic DNA Testing Laboratories
• National Office of Public Health Genomics - (CDC)
• Nuclear Regulatory Commission
• Scientific Working Group on DNA Analysis Methods
• The Secretary's Advisory Committee on Genetic Testing has a draft report U.S. System of Oversight of Genetic Testing:
  A Response to the Charge of the Secretary of HHS , which is 192 pages long, addressing policy issues raised by the development
  and use of genetic technologies and their integration into clinical and public health practice. The home page has the bullets which the report addresses in detail. This report is open for comment until December 21, 2007. This demonstrates the steps being taken to address the moral and ethical issues of genetic testing.
• US Department of Tranportation - Pipeline and Hazardous Materials Safety Administration

Articles

• Lab-on-a-Chip Integration Theme: The Great Challenge
• Beckman-Coulter
• National Cancer Institute Biometric Research Branch - very nice journal articles

Articles

• Lab-on-a-Chip Integration Theme: The Great Challenge
• Beckman-Coulter
• National Cancer Institute Biometric Research Branch - very nice journal articles

Blood Bank

• Approximately half of the erythroblasts in maternal blood are of fetal origin
• Molecular Biology of partial D and weak D: Implications for Blood Bank Practice
• Molecular Genetic Blood Group Typing by the Use of PCR SSP Technique
• Obstetric outcome after RhD and Kell testing
• Overview of Molecular Methods in Immunohematology

Breast Cancer

• Chromogenic in situ hybridization (CISH): a novel alternative in screening archival breast cancer tissue samples for HER-2/neu statu
• Chromogenic in situ hybridization (CISH): A practical method to confirm the immunohistochemical (IHC) results for HER-2 amplification in archival breast carcinoma samples
• Genomics and Molecular Taxonomy of Breast Cancer: Identification of New Prognostic and/or Predicative Factors
• Pathogenesis of breast cancer

Certification

• ASCP Technologist in Molecular Pathology Examination Content Guideline
• Certification of Technologist in Molecular Pathology, MP(ASCP) - Eligibility Guidelines
• ASCP Technologist in Molecular Pathology - Suggested Reading List For Examination Preparation
• NCA Certification Examination for Molecular Biology Specialists - Content outline
• Certified Laboratory Specialist in Molecular Biology [CLSp(MB)] - Eligibility Guidelines

Chromosome Analysis

• Chromonsome Map of Genetic Disorders
• Fish Mapped BACs - Select a chromosome to view the chromosome's reference ideogram and an ordered list of FISH-mapped BACs, with links to CGAP and NCBI related databases. Fascinating! - The Cancer Genome Anatomy Project
• The Cancer Genome Anatomy Project - homepage
• The Cancer Genome Anatomy Project - Educational resources - very nice tutorials

Cystic Fibrosis

• Biochip Based Cystic Fibrosis Carrier Screening - Osmetech Molecular Diagnostics

DNA

• Biology Page has a number of excellent resources illustrating DNA Replication, Sequencing, and Mutation.
• DNA and the Genome - The OpenLearn website gives free access to course materials from The Open University. The LearningSpace is open to learners anywhere in the world.
• DNA Tutorial - 101science.com
• Fingerprinting Methods for Identification of Variable DNA in Genomes
• Replication Of DNA How is cellular DNA copied?
• Review the animations at the McGraw-Hill Biology site
• Virtual Laboratory: DNA Extraction

DNA Sequencing

• Animation - Dolan Learning Center
• DNA Sequencing Core - University of Michigan
• DNA Sequencing - Wikipedia
• How Do We Sequence DNA

Education

• Gen Probe Posters
• Molecular Diagnostic Science Program, The Division of Clinical Laboratory Science , The University of North Carolina at Chapel Hill, Establishing a new clinical site

Enzyme Linked Immuosorbent Assay (ELISA)

• ELISA animation
• ELISA animation
• ELISA animation - prengancy test
• ELISA animation comparing direct and indicrect ELISA
• ELISA Biocrawler

Fluorescent In-Situ Hybridization (FISH)

• An Electronic Textbook on Basic Fluorescent In-Situ Hibrization
• Assessing residual leukemia through fluorescence in situ hybridization (FISH)
• Cytogenetics with special reference to domestic animals - although animals are the primary focus this has very nice basic explanations of FISH
• Fluorescent In Situ Hybridization: (FISH) Created by George Rice, Montana State University
• Fluorescence in Situ Hybridization (FISH) - Science Encyclopedia
• Missing genes - description and pictures of some genetic abnormalities
• What Is FISH - Abbott Laboratories

Forensics

• Forensic Applications of Genetics & DNA
• Forensics Biotechnology
• Quiagen

Gel Electrophoresis

• DNA Gel Electrophoresis
• Gel Electrophoresis of DNA
• Gel Electrophoresis Animation
• Gel Electrophoresis - Animated tutorial Cold Harbor
• Gel Electrophoresis - Proteomics
• Introduction to Recombinant DNA technology Restriction enzymes and gel electrophoresis - restriction endonucleases very nicely done
• Top 10 Fun Facts for DNA Electrophoresis
• Virtual Laboratory: Gel Electrophoresis

Genetics

• An Animated Primer on the Basics of Genes, DNA and Heredity
• DNA Interactive
• Genetic Origins
• Genetics Science Learning Center
• Harry Potter Genetics - powerpoint
• Human Genetics - nice section on ethics, Dave Ussery, Roanoke College
• Human Pedigree Analysis - autosomal recessive and dominant examples
• Human Pedigree Analysis 1
• Learn Genetics
• Mendelian Inheritance - X-Linked Inheritance
• My DNAi
• Pedigree Analyis - powerpoint
• Pedigree: Working Out Inheritance Patterns - powerpoint
• Real-world Genetics

Glossary

• A Glossary Of Terms Commonly Used In Molecular Biology
• Glossaries of Genome / Human Genetics Terms - list of glossary sites
• Glossary of Terms Used in Molecular Genetics
• A Molecular Biology Glossary - A Quick and Dirty Reference to Terms Used in Molecular Biology
• DNA Heritage
• Gene Links - University of Washington
• Glossary of Biochemistry and Molecular Biology
• Glossary of Molecular Biology Terminology - scroll down page to see glossary

Hybridization

• Hybridisation - includes microarrays, comparative genomic hybridisation and FISH
• Hybridization: Theory and Practical Aspects
• Nucleic Acid Hybridizations

Meiosis and Mitosis

• Meiosis - Animated Tutorial
• The Cell Cycle & Mitosis Tutorial
• Overview of Meiosis

Microarray

• Assay: Protein Microarrays on Nitrocellulose SlidesAssay: Protein Microarrays on Nitrocellulose Slides
• Demystified … Tissue microarray technology
• DNA Microarray - animated tutorial
• Introduction to DNA microarray technologies
• Introduction to Microarray Technology
• Macroarray versus Microarray - end of presentation (about slide 75) more basic
• Microarray animation
• Microarray - list of links with tutorials and animations
• Microarray Tutorial
• Microarray Technologies ppt
• Tissue Microarrays: A Current Medical Research Tool
• Virtual Laboratory: DNA Microarray

Newborn Screening

• Development of Matched Phenotypic and Genotypic Control Materials in Dried Blood Spots

Newsletters/ News Sites

• Lab On A Chip

Oncology

• Assessing residual leukemia through fluorescence in situ hybridization (FISH)
• Biomarkers: The Clues to Genetic Susceptibility
• Cancer and the Cell Cycle
• General Characteristics of Cancers - very nice graphics
• Genetic alteration and gene expression modulation during cancer progression - 2004
• Clinical and Molecular Oncology
• Department of Radiology/Molecular Epidemiology - mouse over "Survey of Mutations in Oncogenes and Tumor Suprresor Genes" pictures
• Oncogenes: The (Autosomal) Dominant Evil 2003
• Pathogenesis of breast cancer

Pedigrees

• How to Draw Your Pedigree
• Human Pedigree Analysis
• Human Pedigree Genetics Problem Set
• PediDraw: A web-based tool for drawing a pedigree in genetic counseling
• Human Pedigree Analysis 1

Pharmacogenetics

• Roche
• Streamlining Pharmacogenetic Analysis - A person’s genetic make up can have a profound influence on how he or she will respond to a drug

Polymerase Chain Reaction (PCR)

• PCR Animation Links
• PCR for Dummies
• PCR Tutorial for Absolute Beginners
• Protocol for PCR with Taq DNA Polymerase
• The World Famous PCR Jump Station: Polymerase Chain Reaction
• Scorpions Reaction Scorpions™ is a homogeneous or closed-tube platform for PCR analysis.

PowerPoint Presentations

• Biochemistry Basics - list of powerpoint presentations
• Molecular Diagnosis of Bacterial Infections: Current Status and Future Trends
• Use of Molecular Methods for Diagnostics of Infectious Diseases

Primer Design

• Primer Design - short tutorial which includes the 7 basic rules for primer design.
• PCR Primer Design Guidelines Premier Biosoft
• Integrated DNA Technologies - Wonderful site to create your own primers.

Proteomics

• Proteomics Technologies

Restriction Fragment Length Polymorphisms (RFLPs)

• Self Quiz Ch.20: DNA Technology - Quiz
• Recovery of DNA from Aged Bloodstains
• RFLP animation
• RFLP Wikipedia
• RFLP Computationaal Biology - Nice overview of the procedure.
• The Case of the Crown Jewels: Investigate a Crime Scene Using DNA Restriction Analysis (DNA Fingerprinting)

RNA

• Anti-Sense RNA
• DNA, RNA, and Protein Synthesis - be sure to take the quiz for each unit
• Genetic Code - explanation of sense and anti-sense strand
• Introns - Widipedia
• Introns and Exons
• Nucleic acid structure and function - nice descriptions of the different types of RNA
• Polyadenylation - Wikipedia
• RNA - Wikipedia
• Template versus Coding Strand
• The Functions of Introns: From Junk DNA to Designed DNA
• Transcription Tutorial
• Transcription Animation

Solution Hybridization

• New Tools for miRNA and siRNA Analysis
• Isolation and Detection of Small RNA Molecules - powerpoint Ambion

Temperature of Melting

• Comparison of different melting temperature calculation methods for short DNA sequences
• Description of the methods for DNA/DNA melting temperature calculations
• DNA Melting - Wikipedia
• HYBRIDIZATION NOTES - Melting and Annealing Temperature - Dr. Bart Frank, Arthritis and Immunology Program, OMRF
• Melting Temperature of Oligonucleotides - Sigma Aldrich
• Online Melting Temperature (Tm) Calculation
• Thermodynamics of DNA Duplex Denaturing - MSU

Testing - Sites with Lists of Testing

• A Science Primer - NCBI
• Biochem4schools
• Case Studies in Cell and Molecular Biology for post-16 Students
• Clinical Molecular Diagnostic Laboratory -
• Department of Laboratory Medicine, Molecular Diagnostics, Yale University
• Molecular Diagnostics - University of Pittsburg Medical Center
• Molecular Diagnostics Laboratories - Cincinnati, Ohio
• Molecular Diagnostics Laboratory - MD Anderson

Tuberculosis

• Automated DNA Fingerprinting Analysis of Mycobacterium tuberculosis Using Fluorescent Detection of PCR Products
• Molecular Epidemiology of Mycobacterium tuberculosis Strains Isolated from Different Regions of Italy and Pakistan
• Rapid Discrimination of Mycobacterium tuberculosis Complex Strains by Ligation-Mediated PCR Fingerprint Analysis

Tutorials

• Bio-Body Guard
• BIOL 114 Genetics and Development
• Biology I Animations Movies & Interactive Tutorial Links
• DNA Anatomy
• DNA Microarray Flash Tutorial
• Education Resources — Graphics, Animations, Tutorials, and More - BioRad
• ELISA animation
• ELISA animation
• ELISA animation - prengancy test
• ELISA animation comparing direct and indicrect ELISA
• Gel Electrophoresis Animation
• Gen Probe - Earn FREE CEUs
• Genome Analysis: Microsatellites or SNPs
• Get a Clue
• Introduction to DNA microarray technologies
• John Hopkins Presentations
• John Hopkins Molecular Pathology Diagnostics Laboratories Assay Tutorials
• Microsatellite DNA Methodology
• Microarray Technologies ppt
• Microarray Tutorial
• Microarray animation
• Molecular Diagnostics Web Links - University of North Carolina at Chapel Hill
• Molecular Searching Techniques - overview of Western, Southern, and Northern blots, FISH, RFLP
• PCR Animation Links
• RFLP animation
• Scorpions Reaction Scorpions™ is a homogeneous or closed-tube platform for PCR analysis.
• Understanding Cancer Series: Molecular Diagnostics - National Cancer Institute

Western Blot

• Western Blot -
• Southern, Northern, Western, and South-Western Blots

Sites with Educational Links

• Biofluorimetry Lab
• Biology - Alief ISD, Houston, TX
• Cytogenetic Resources
• Genomic Medicine - open source courseware from MIT, audio lectures
• Graphics Gallery - Looking for graphics? The Graphics Gallery is a series of labeled diagrams with explanations representing the important processes of biotechnology.
• Introduction to Human Genetics 2005 - Jonathan Wolfe, London's Global University
• www.101science.com

Last Update: January 14, 2012
Web Author: Terry Kotrla MT(ASCP)BB
Comments: kotrla@austincc.edu
Copyright ©2007 by Terry Kotrla - All Rights Reserved