MLAB 2378 Fundamentals of Molecular Diagnostics Links

MLAB 2378 Fundamentals of Molecular Diagnostics

Unit 17 Objectives - DNA Based Tissue Typing

State the importance of the human leukocyte antigens in transplantation.
List and describe the three classes of HLA genes.
Compare and contrast the terms "polymorphism" and "mutation".
Compare and contrast the levels of typing resoultion that are achieved by different laboratory methods.
List and describe the methods used for anlaysis of HLA alleles in the HLA laboratory for typing, screening and crossmatching.
List three advantages of serological HLA typing procedures.
Describe the principle and procedure of the complement dependent cytotoxicity test.
State the significance of panel reactive antibodies (PRA) and how this influences donor selection.
Describe each of the following HLA testing procedures: Sequence specific oligonucleotide probe hybridization (SSOP), Sequence specific PCR (SSP), and Sequence based testing (SBT).
List three molecular based testing which may be utilized for HLA typing but are not routine methods used in the clinical laboratory.
State the advantages for performing serological and DNA based testing.
Describe three causes of discrepant results in HLA typing.
State the level of resolution for HLA typing required for: solid organs, stem cell and bone marrow transplants.
State the clinical significance of minor histocompatibility, nonconventional MHC antigens and killer cell immunoglobulin-like receptors.
List four diseases which are associated with particular HLA haplotypes.
Discuss the significance of HLA haplotypes associated with disease and the diagnostic value.
Define or describe the terms listed in the "Unit 17 Vocabulary".

Molecular Typing in Transplant Diagnostics

Human Leukocyte Antigen (HLA) testing is used to determine compatibility between a patient and donor for solid organ and bone marrow transplantation.

A variety of methodologies are available to determine HLA type depending on resolution and throughput needs.

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