Unit 7 Objectives - Resolution and Detection of Nucleic Acids
Explain the principle and performance of electrophoresis as it applies
to nucleic acids.
Compare and contrast agarose and polyacrylamide gel polymers.
State the principle of electrophoresis.
Explain the performance, advantages and disadvantages of each of the
following electrophoretic methods: traditional, pulsed field gel and
capillary as it is applies to nucleic acid separation
Describe the general types of reagents and equipment used for electrophoresis.
Compare and contrast detection systems (Ethidium Bromide, SyBr green
and silver stain) used in nucleic acid applications.
