Introductory note: PCR is like a xerox machine for DNA, your copy
is the DNA, and the toner, image capture software and paper are the
elements used to make exact copies, from 1 to a million or more. The
number of copies depends on the amount of supplies you have loaded into
the machine, just as the number of copies of DNA is dependent on the
amount of reagents you put in the mix. It's really a very simple concept.
The application of PCR can be a lot more complicated.
Instructor Overview
- I'm not quite done with it, still need to add some information about
very last section of chapter but please use it to start your studying.
Powerpoint
- Donna C. Sullivan, PhD, Division of Infectious Diseases, University
of Mississippi Medical Center
There is so much excellent information out there on PCR, it is
very difficult to pick the "best of the best". I have provided
you with links for the Notes and Assignments sections but do not be
afraid to go out on your own. If you find an exceptional site, send
it to me. The following are "must see" sites.
PCR
tutorial video - very basic explanation and animation of PCR. View
the "narrated" animation.
