MLAB 2479 Molecular Diagnostics Techniques
 

MLAB 2479 Molecular Diagnostics Techniques (4-3-4)

Course Description

An introduction to the theory and laboratory techniques in molecular biology with an emphasis on molecular diagnostics, including DNA replication, transcription, gene expression and regulation, recombinant DNA and RNA techniques. Topics include hybridization techniques and amplification techniques such as QPCR. Fee: $24 Insurance: $3.50Skills: P Prerequisites: MLAB 2378. Course Type: W

Prerequisites: Graduation from an accredited MLT/CLT or MT/CLS program or Department Chair approval. Submission of a completed application for the Molecular Diagnostics certificate program and successful completion of a Criminal Background Check.

Course Goals

  1. Apply knowledge of cellular structure and function, especially DNA and RNA, to molecular diagnostic procedures.
  2. Gain a thorough working knowledge of nucleic acid extraction, resolution and detection.
  3. Gain a solid foundation in the most commonly utilized molecular diagnostic testing protocols.
  4. Apply the knowledge of molecular testing to the most commonly performed applications in the clinical laboratory such as: nucleic acid extraction, resolution and detection, analysis and characterization of nucleic acids and proteins, nucleic acid amplification and DNA sequencing.

Course Objectives

  1. Identify the important parameters in the design of a laboratory to conduct the most commonly-used molecular diagnostics protocols.
  2. Identify the important parameters in the design of a quality system for molecular analyses.
  3. Become proficient with the techniques required in order to perform the most commonly-used molecular diagnostics protocols.
  4. Identify the important parameters in the design of a molecular diagnostic test.
  5. Identify the components of a well-controlled diagnostic test.
  6. Use critical thinking skills to trouble shoot problems as they occur and determine possible causes.

Last Update: February 12, 2009
Web Author: Terry Kotrla, MS, MT(ASCP)BB
Original publication date: January 2008
Comments: kotrla@austincc.edu