MLAB 2479 Molecular Diagnostics Techniques (4-3-4)
An introduction to the theory and laboratory techniques in molecular
biology with an emphasis on molecular diagnostics, including DNA replication,
transcription, gene expression and regulation, recombinant DNA and RNA
techniques. Topics include hybridization techniques and amplification
techniques such as QPCR. Fee: $24 Insurance: $3.50Skills: P Prerequisites:
MLAB 2378. Course Type: W
Prerequisites: Graduation from an accredited MLT/CLT or MT/CLS program
or Department Chair approval. Submission of a completed application
for the Molecular Diagnostics certificate program and successful completion
of a Criminal Background Check.
- Apply knowledge of cellular structure and function, especially DNA and RNA, to molecular diagnostic procedures.
- Gain a thorough working knowledge of nucleic acid extraction, resolution and detection.
- Gain a solid foundation in the most commonly utilized molecular diagnostic testing protocols.
- Apply the knowledge of molecular testing to the most commonly performed applications in the clinical laboratory such as: nucleic acid extraction, resolution and detection, analysis and characterization of nucleic acids and proteins, nucleic acid amplification and DNA sequencing.
- Identify the important parameters in the design of a laboratory
to conduct the most commonly-used molecular diagnostics protocols.
- Identify the important parameters in the design of a quality
system for molecular analyses.
- Become proficient with the techniques required in order to perform the
most commonly-used molecular diagnostics protocols.
- Identify the important parameters in the design of a molecular
- Identify the components of a well-controlled diagnostic test.
- Use critical thinking skills to trouble shoot problems as they occur and determine possible causes.
Last Update: January
Web Author: Terry Kotrla, MS, MT(ASCP)BB
Original publication date: January 2008