WPCi% ]iPiLڔ5`^ ;CVZP`z8d86KU-s:g$$$$$$$$$$$$$$$$$$$$$$$$$$$$ B2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2%2% CO%HPE438C7 (HP Officejet J6400 series)0(9 Z6Times New Roman RegularX($USUS.,7#137=CIQYag1.a.i.(1)(a)(i)1)a)(9 Z(Times New Roman y|=  `,=kupBACKUP3|x+U(UhCEKQW]cioAutoList31.1.1.1.1.1.1.1.(5hCEKQW]cioAutoList11.1.1.1.1.1.1.1.(;3$2#  0  .3  0  (O;$0  2#  a  .3  0` (#(#3#37=CIQYag1.a.i.(1)(a)(i)1)a)i)i) ? 'USUS.,  _Laboratory13: MononucleosisTesting .(#_MLAB_Ԁ1335Immunology/Serology  HHAY) xdEHH!A  (.3$ !USUS.,  \(EhCEKQW]cioAutoList21.1.1.1.1.1.1.1.TABLE B8 CSY_ekqw}Numbers 2Numbers1.1.1.1.1.1.1.1.1.\TABLE A*+ (_2623  ..*D+D (_25   ," <DL,23  ..," <DL,   d*5+5 (_24  ) <DL)23  ..) <DL)  *2+2 (_23 ` &<<DL&23  ..&<<DL& ` */+/ (_22  #DL#23  ..#DL#  *,+, (_21   DL 23  .. DL  *)+) (_20 h DDL23  ..DDL h *&+& (_19  L23  ..L  *#+# (_18   L23  .. L  *>> (_17  2( 4 <DL223  Ԁ2( 4 <DL2  *DD (_16   ," <DL,23  Ԁ," <DL,   *55 (_15  ) <DL)23  Ԁ) <DL)  *22 (_14 ` &<<DL&23  Ԁ&<<DL& ` *// (_13  #DL#23  Ԁ#DL#  *,, (_12   DL 23  Ԁ DL  *)) (_11 h DDL23  ԀDDL h *&& (_10  L23  ԀL  (## &_9   L23  Ԁ L  (>> &_8  2( 4 <DL223  2( 4 <DL2  (DD &_7   ," <DL,23  ," <DL,   (55 &_6  ) <DL)23  ) <DL)  (22 &_5 ` &<<DL&23  &<<DL& ` (// &_4  #DL#23  #DL#  (,, &_3   DL 23   DL  ()) &_2 h DDL23  DDL h (&& &_1  L23  L  &## $_   L23   L  <:Default Para\  `&Times New Roman dd !USUS.,  _XXXX("__8XXdd8 Exercise13: &%XXԀ MononucleosisTesting    Objectives:   "3"  3u2#  1  .3  0    Followinstructionsofthereagentpackageinserttoselectandevaluateappropriatespecimensfor 1 t Mononucleosistesting.3u݌ L(#(# Ќ  "3"  32#  2  .3  0    Performthemononucleosiskittestforthedetectionofantibodiestoobtaincontrolandpatient  $ resultsthatmatchinstructorvalueswith100%accuracy.3݌ (#(# Ќ  "3"  3A2#  3  .3  0    Evaluatereagentpackageinsertstodetermineeachofthefollowing:3An݌ (#(# Ќ  ";"  ;N0  2#  a  .3  0` (#(#  thesubstancebeingdetected.;N{݌i ` (#` (# Ќ  ";"  ;H0  2#  b  .3  0` (#(#  theprincipleoftheprocedure.;Hu݌A ` (#` (# Ќ  ";"  ;E0  2#  c  .3  0` (#(#  sampleswhichareacceptablefortesting.;Er݌\ ` (#` (# Ќ  ";"  ;K0  2#  d  .3  0` (#(#  theexpectedresultifthesubstanceispresent.;Kx݌4 ` (#` (# Ќ  ";"  ;Z 0  2#  e  .3  0` (#(#  clinicalsignificanceofpositiveresults.;Z ݌ ` (#` (# Ќ  ";"  ;a 0  2#  f  .3  0` (#(#  limitationsoftheprocedure.;a ݌ ` (#` (# Ќ  ";"  ;[ 0  2#  g  .3  0` (#(#  troubleshootingprocedurestofollowwhencontrolresultsareunacceptable.;[ ݌y ` (#` (# Ќ  "3"  3 2#  4  .3  0    Accuratelyrecordandreportresultsasinstructed.3 ݌Q (#(# Ќ  "3"  3 2#  5  .3  0    Utilizelecturenotes,textbookandlaboratory(includingproductinserts)informationtoanswer )l studyquestions.3 ݌D(#(# Ќ  Introduction:   #XX%&##XXXX=#<( 4 <DLX<=XXXX=&%X=XInfectiousmononucleosis(IM)isanacuteorsubacutebenigninfectious_lymphoproliferative_Ԁdiseasethat a isassociatedwiththeEpstein#=XX%=&㞘#=&%X=XBarrvirus(_EBV_).Thediseasemostoftenaffectschildrenandyoung 9| adultsandischaracterizedbyanincreaseinatypicallymphocytes,enlargementofthelymphnodesand T spleen,feverandproductionofcharacteristicheterophileantibodies.TheresultsoftheIMtestshouldbe , usedbythephysicianalongwithclinicalandotherlaboratorydata,suchasaCBCwiththepresenceof  Downeycells,tocometoadiagnosisofIM.    #=XX%=&㞖#=&%X=XSerologicaltestingformononucleosisbecameavailableasaresultoftheresearchofPaulandBunnellon I heterophileantibodies.Heterophileantibodiesarethosethatwillreact(crossreact)withanymemberof ! d agroupofsimilarantigensthatcanbefoundinunrelatedanimalsormicroorganisms.Theantigens,  < calledheterophileantigens,aresosimilarthatanantibodybuilttoonewillcrossreactwiththeothers. !  PaulandBunnellnotedthatduringthecourseofinfectiousmononucleosis,patientsdeveloped "! heterophileantibodieswhichwerecapableofagglutinatingerythrocytesfromsheepandhorses. #" DavidsohnmodifiedtheoriginalPaul#=XX%=&#=&%X=XBunnelltestbyaddingstepstoabsorboutcrossreacting_Forssman_ Y$# andserumsicknessantibodiesleavingbehindtheIMheterophileantibodytoreactwithsheepredcells. 1%t$ Theiradaptation,theDavidsohnDifferentialtest,becametheclassicalreferencemethodforlaboratory  &L% diagnosisofinfectiousmononucleosis,howeveritistimeconsumingtoperform. &$ & #=XX%=&Z#=&%X=XRecentresearchershavedevelopedtestsforinfectiousmononucleosisheterophileantibodiesusing (!( treatedhorseorbovineredcells,orextractedantigensfrombovine_RBCs_Ԁthathavebeenattachedtolatex i)") particlesorcoatedintubesforenzyme#=XX%=&r#=&%X=Xlinkedimmunoassaytesting(EIA). A*#* #=XX%=&##XXX=Xy#&%XX&%%& +4%, e Principle:    Reviewtheprincipleofthespecifickitbeingusedinthislaboratory.  m  Materials :  ` ! ! (#33"3"  3?2#  1  .3  0    Mononucleosistestingkit3?l݌ (#(# Ќ  "3"  3#2#  2  .3  0    Patientandcontrolserumspecimens.#XX%&㞌#&%XX#&%%&㞫#&%%&3#P݌ (#(# Ќ  "3"  32#  3  .3  0    Timer3݌ (#(# Ќ  "3"  3b2#  4  .3  0    Othermaterialsasdirectedbyreagentproductinsert.3b݌Y (#(# Ќ   (5h83 Procedure:   L  Seespecificreagentproductinsert.    Interpretation:  i  Seespecificreagentproductinsert. \  ExpectedResults:    1.0  Consultthereagentproductinsertforinterpretation.y(#(# 2.0  Aswithalltestsforheterophileantibodies,falsepositiveandnegativeresultsdooccur,but Q usuallyinlownumbers(approximately1%).)l(#(# 3.0  Detectableheterophileantibodylevelmaybeobservedasearlyasfourdays,butmorecommonly D requiresthreeweeksormore.Somepersons,especiallyyoungchildren,willnotproduce  observableheterophileantibodylevelsalthoughclinicalandhematologicalsignsareevident.(#(# 4.0  Positivetestresultsmaypersisteventhoughclinicalsymptomsareabsent.(#(#  Precautions:  9| 1.0  Followmanufacturersdirectionsexactly(unlessdirectedbyinstructor). ,(#(# 2.0  Reagentsandspecimensmustbeatroomtemperature.! (#(# 3.0  Reviewtheproductinsertthespecifickitbeingusedinthislaboratoryforadditional "! information. u#"(#(#   M$# _ @ Laboratory13:MononucleosisTesting   @ResultsandStudyQuestions  Name_____________________________________1(#Date_____________________ E  *Yddd Xdd Xdd Xe(#e(#,xdd +   8 8 TestKitName_____________________________________ 'Y  'Manufacturer0 y _____________________________________ 1'" y y"y y" 1LotNumber0 y _____________________________________ 1'"1ty y"y y" 1ExpirationDate_____________________________________(    ( Interpretresultsas posforpositiveor negfornegative.  }  *eYddxdd xYe(#e(#e,*dd ,N dd +  &"-p -p  &@ z PatientNameandIdentificationNumber /" " /@:Result D: "  ?1.?D1.0 ! >42@!+!+  ?1. ? > 5+ @  @2.@52. 4*(i  @2. @ 4 " i @  z Controls /"" / $" $Positive  A   A Negative          Usingyourtextbook,lectureandlabresultsandnotes,answerthefollowingquestions.Eachquestionis  worthonepoint,unlessotherwiseindicated.   ( 98"3"  3#/2   1  .3  0    Basedonthecontrolresults,canthesepatientresultsbereported?3#/P/݌E (#(# Ќ    YesNo (circleone)0 Ifnot,explainwhy. ` (# (# "3"  302   2  .3  0    Whatisthecarrierparticleintheindicator/testsystem(towhatsubstanceistheantigens   physicallyattached)?300݌ (#(# Ќ  "3"  312   3  .3  0    Whatspecimensmaybetestedbythisprocedure?312݌ L (#(# Ќ  "3"  322   4  .3  0    Stateatleastthreeconditionswhichmaycausepositiveresultsforheterophileantibodies.(1.5 i  points)323݌A (#(# Ќ  "3"  3342   5  .3  0    Whatisthegenusandspecieswhichisthecausativeagentofinfectiousmononucleosis?334`4݌Q(#(# Ќ  "3"  3V52   6  .3  0    WhatfactorsmustbeconsideredbeforeadiagnosisofIMcanbemade?(1.5points) @ 3V55݌(#(# Ќ  "3"  362   7  .3  0    Afterreviewingtheproductinsertstatethe principle ofthistestkitincludingtheappearanceof  , positiveandnegativereactions.(Inyourownwords,please.)366݌! (#(# Ќ  #XX%&##XXXX2#