BIOLOGY 1406 Course Objectives

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BIOL 1406 (formerly BIO 1654) is an in-depth study of cellular and molecular biology and is designed for science majors and students with a strong science background who desire a more in-depth approach to biological topics. As summarized in the textbook, we will be covering three major topics: The Chemistry of Life (Atoms, Molecules, Macromolecules, and Metabolism); The Cell (Structure and Function, Energetics, Cell Cycle); Genetics (Mendelian, Chromosomal and Molecular Genetics, Prokaryotic and Eukaryotic Gene Expression and Regulation, DNA Technology)

Common Course Objectives

Introduction to Biology
1. Discuss scientific processes and show how it is used in the study of biology.
2. Differentiate between a hypothesis, a theory and a law.
3. Identify some of the limitations of science.
4. Distinguish between living organisms and nonliving matter.
5. Define evolution, give examples of the evidence for evolution and how it is a unifying concept for biology.

Basic Chemistry and Biochemistry    Back to Top
1. Describe the basic structure of the atom.
2. Interpret chemical and structural formulas.
3. Compare ionic and covalent bonds and describe their characteristics.
4. Discuss hydrogen bonds and non-polar interactions, and their importance for living organisms.
5. Identify the characteristics of carbon that allow it to play such an important role in the chemistry of life.
6. Explain isomers in biology.
7. Describe the properties of water and explain their importance to living systems.
8. Explain properties of acids, bases and salts.
9. Explain roles buffers play in living organisms.
10. Understand pH.
11. Identify the functional groups of biological molecules.
12. Describe structures and functions of carbohydrates, lipids, proteins and nucleic acids.
13. Describe the four levels of protein structure and how they relate to protein activity.

Introduction to Cell Structure and Function    Back to Top
1. Describe the cell theory.
2. Explain some factors that limit cell size.
3. Describe the typical prokaryotic cell including size, plasma membrane, cell wall, cytoplasm, ribosomes, nucleoid and flagella.
4. Describe the typical eukaryotic cell and identify the structure and function of: nucleus and nucleolus, endomembrane system, ribosomes, mitochondria, plastids, the cytoskeleton and cilia, flagella and centrioles.
5. Compare and contrast the characteristics of prokaryotes, eukaryotes and viruses.
7. Discuss diversity among eukaryotic cell types.

Membranes and Cell Transport    Back to Top
1. Describe the fluid mosaic model of membrane structure and explain the structure and functions of component molecules.
2. Compare and contrast diffusion, osmosis and dialysis.
3. Explain cell transport of large and small molecules across biological membranes.
4. Discuss cell signaling mechanisms including the role of cAMP

Principles of Cell Metabolism    Back to Top
1. Distinguish between potential and kinetic energy.
2. State the first and second laws of thermodynamics and explain how they apply to living organisms.
3. Explain what a chemical reaction is and describe changes that take place during a chemical reaction.
4. Distinguish between endergonic and exergonic reactions and explain how they are coupled in living organisms. Describe the roles of ATP and ADP in the coupling of chemical reactions.
5. Describe the structure, how enzymes work, regulation of enzyme activity ( e.g. cofactors and inhibitors) and factors that affect enzyme activity.
6. Describe biochemical pathways, substrates, products and intermediate products.

Photosynthesis    Back to Top
1. Explain the relationship between structure and function of chloroplasts.
2. Describe the light dependent reaction.
3. Describe the light independent reaction.
4. Summarize the process of photosynthesis and trace movement of carbon dioxide and water through the process.

Respiration and Fermentation    Back to Top
1. Describe the process of glycolysis and fates of pyruvate.
2. Explain relationships between structure and function of mitochondria.
3. Describe citric acid cycle and oxidative phosphorylation.
4. Summarize the process of aerobic respiration. Trace the fate of glucose and the role of oxygen in the process.
5. Discuss energy capture and release under anaerobic conditions( e.g. alcohol and lactic acid production).
6. Compare and contrast aerobic and anaerobic processes.
7. Explain how other biomolecules can enter these biochemical pathways

Cell Cycles    Back to Top
1. Describe the process of binary fission in a prokaryotic cell.
2. Discuss eukaryotic cell cycle and describe the events of interphase.
3. Describe mitosis.
4. Describe cytokinesis and distinguish between mitosis and cytokinesis. Also distinguish between the cytokinesis of plant and animal cells
5. Describe meiosis I and II.
6. Compare and contrast mitosis and meiosis.

Patterns of Inheritance    Back to Top
1. Define and be able to use the following terms correctly: genotype, phenotype, homozygous, heterozygous, alleles and genes
2. Discuss Mendelian model of inheritance.
3. Describe patterns of inheritance (e.g. sex linkage, dominant, recessive, codominant and incomplete dominant).
4. Describe genotypic and phenotypic variation.
5. Discuss chromosomal variations in humans.

DNA Structure and Replication    Back to Top
1. Describe the structure of nucleotides.
2. Describe the DNA molecule.
3. Describe DNA replication, including the principle steps and enzymes involved.
4. Discuss the structure of eukaryotic and prokaryotic chromosomes.
5. Discuss DNA repair mechanisms.
6. Define mutation and give examples (e.g. point mutations, chromosome changes).

Protein Synthesis    Back to Top
1. Describe the structure of RNA.
2. Compare and contrast RNA and DNA.
3. Describe transcription including the principle steps and enzymes involved.
4. In detail, describe the process of translation, identifying the principles steps.
5. Describe posttranscriptional modifications of mRNA in eukaryotic cells.
6. Decrribe protein processing and distribution.
7. Use base pairing rules to replicate a segment of DNA, transcribe it and match the anticodon of tRNA to mRNA. Translate a segment of mRNA using a genetic table.
8. Explain how various types of mutations can alter the structure of a polypeptide chain.

Gene Regulation    Back to Top
1. Describe the process of gene regulation in prokaryotic cells (operon system).
2. Describe gene regulation strategies in eukaryotic cells.

Virus Structure and Replication
1. Describe the structure of viruses and how they are replicated

Genetic Manipulation    Back to Top
1. Compare artificial genetic recombination to natural processes.
2. Describe plasmid and virus vectors.
3. Describe recombinant DNA and cloning techniques.
4. Describe PCR and its utility.
5. Discuss application biotechnology (e.g. RFLPs, VNTR, DNA fingerprinting).