MLAB 2321 Molecular Diagnostics For Clinical Laboratory Science
This course provides a comprehensive overview of the fundamental principles
of clinical molecular diagnostics and explores the use of molecular techniques
in the diagnosis of disease. Topics to be covered include: nucleic acid
structure and function, genetics, DNA chemistry, introduction to nucleic
acid isolation, identification and amplification techniques used in infectious
disease diagnosis in the clinical laboratory, components of a molecular
laboratory, and evaluation of controls to validate results obtained.
Prerequisites: Graduation from an accredited MLT/CLT or MT/CLS program
or Department Chair approval. Submission of a completed application
for the Molecular Diagnostics certificate program and successful completion
of a Criminal Background Check.
- Apply knowledge of cellular structure and function, especially DNA
and RNA, to molecular diagnostic procedures.
- Gain a thorough working knowledge of nucleic acid extraction, resolution
- Gain a solid foundation in the most commonly utilized molecular diagnostic
- Apply the knowledge of molecular testing to the most commonly performed
applications in the clinical laboratory such as: nucleic acid extraction,
resolution and detection, analysis and characterization of nucleic acids
and proteins, nucleic acid amplification and DNA sequencing.
- Describe cell function and structure.
- State the basic principles of modern genetics as they apply to disease
- State the structure, function and duplication of DNA and RNA.
- Describe methods for nucleic acid extraction and detection.
- List and describe the methods for analysis and characterization of
nucleic acids and proteins.
- Describe chromosomal structure and mutations and methods to detect.
- Briefly describe techniques used in the clinical lab to detect: DNA
Polymorphisms, microorganisms, inherited diseases, neoplastic diseases
and DNA-based tissue typing.
- State the required quality assurance and quality control measures
required in a molecular laboratory.
Last Update: November 15, 2012
Web Author: Terry Kotrla, MS, MT(ASCP)BB