MLAB 2321 Molecular Diagnostics for Clinical Laboratory Science

Unit 4 Objectives - RNA

  1. State the "central dogma" relating to nucleic acid function. (Found in the PowerPoint in the notes section)
  2. Describe the composition of Ribonucleic acid (RNA).
  3. Compare and contrast the structure of RNA with that of DNA.
  4. List and compare the three primary types of RNA including where they are found in the eukaryotic cell.
  5. Describe the cellular processing of messenger RNA.
  6. Describe how ribonucleotides are polymerized into RNA (transcription) and the relation of the sequence of the RNA transcript to the DNA sequence of its gene.
  7. Recognize the reactions catalyzed by ribonucleases and RNA helicases and their roles in RNA metabolism.
  8. State the two factors responsible for RNA synthesis.
  9. State the direction of RNA polymerization.
  10. State the purpose of “capping”.
  11. Describe the process and purpose of polyadenylation.
  12. State the purpose of splicing.
  13. Describe the process of transcription.
  14. Describe the process of translation.
  15. List several types of RNA polymerases, their substrates and products.
  16. Be able to define or describe the terms listed in the "Unit 4 Glossary of Terms".

Last Update: March 6, 2015
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