Unit 8 Objectives- Analysis and Characterization of Nucleic Acids
Describe how restriction enzyme sites are mapped on
Compare and contrast Northern, Southern, and Western
blot hybridzation methods including the target, probe and purpose.
List and describe the three methods used to tranfer the fragments
separated by electrophoresis to a solid medium.
State the purpose of baking or UV cross-linking the denatured DNA
after tranferring to the membrane.
State the purpose of the prehibridization step in the Southern blot
Calculate the melting temperature of a given sequence
Describe the basic method and uses of the following
hybridization technologies: dot blot, slot blot, reverse dot blot,
macroarray, microarry, high density oligonucleotide array and microelectronic