MLAB 2321 Molecular Diagnostics for Clinical Laboratory Science

Unit 9 Assignments- Nucleic Acid Amplification

Submit the G/C Content Calculation and Temperature of Melting to the Assignments section of BlackBoard

Tutorial - NOT Optional.

  1. G/C Content Calculation Tutorial - please review, practice and be prepared to calculate G/C content on the exam. Reference: GC - Content Wikipedia
  2. Temperature of melting - please review, practice and be prepared to calculate the Temperature of Melting on the exam. Reference: DNA Melting Wikipedia

Review the Following Web Sites:

  1. Polymerase Chain Reaction - tutorial with animations
  2. Real Time PCR - this site goes from basic to advanced, focus on the basic concepts.


  1. Nucleic Acid-Amplification Testing Further Safeguards Nation's Blood Supply, NHLBI Study Shows
  2. Recommendation Statement Screening for Gonorrhea U.S. Preventive Services Task Force

Last Update: May 7, 2014
Web Author: Terry Kotrla, MS, MT(ASCP)BB
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