MLAB 2337 Molecular Diagnostics Techniques
 

MLAB 2337 Molecular Diagnostics Techniques
Schedule Summer 2015
11 Week Session: June 1 - August 16
The schedule below is TENTATIVE and subject to change.

Required Textbook: Molecular Diagnostics: Fundamentals, Methods and Clinical Applications, 2nd edition. Authors: Lela Buckingham and Maribeth Flaws. ISBN-13: 978-0-8036-2677-5
Dates
Textbook Reading Assignments
Assignments and Laboratory Activity
June 1 - 7 Review Chapters 1 and 2 Syllabus Statement of Understanding Quiz - Deadline Sunday, June 7, 2015
Genetics Assignment
June 8 - 14 Review Chapters 3, 4 and 5 DNA/RNA Assignment
June 13
Day 1
ON CAMPUS
Review Chapter 18 Completion of Certificate - Clinical

Print out the following documents before coming to lab:

Lab 1. Laboratory Safety
Lab 2. Design of a Molecular Diagnostic Lab
Lab 3. Micropipettes
Lab 4. Quality Assurance in the Molecular Laboratory & Validation of Micropipettes
Upload into Assignments Day 1 in BlackBoard - Due Date Sunday, June 21, 2015

IMPORTANT: Please go into BlackBoard to review reference documents for Labs 2 and 3. Be sure to watch the video on the proper use of a micropipette BEFORE coming to lab.
June 15 - 21 Review Chapter 6
  1. Review information presented in units 3, 6 and 7 http://www.austincc.edu/mlt/mdfund/mdfund_schedule
  2. Review the “Gel Electrohoresis Virtual Lab” http://learn.genetics.utah.edu/content/labs/gel/
  3. Review Gel Electrophoresis from the DNA Learning Center:  http://www.dnalc.org/resources/animations/gelelectrophoresis.html
  4. Review Experiment 2: Gel Electrohoresis of DNA ”virtual lab:  http://www.life.illinois.edu/molbio/geldigest/electro.html
  5. Review Separating Fragments of DNA by Gel Electrophoresis” http://www.sumanasinc.com/webcontent/animations/content/gelelectrophoresis.html
June 22 - June 28 Review Chapters 7 and 8
  1. Review information presented in units 8 and 9 http://www.austincc.edu/mlt/mdfund/mdfund_schedule
  2. Review "DNA Extraction Biotechniques Virtual Lab"
  3. Review Kary Mullis' videos on PCR
  4. Watch "DNA Probe" and take the quiz.
  5. Watch "Southern Blot" presentation and take the quiz.
  6. Perform a Virtual PCR.
Exam 1 Covers Labs 1-4, Deadline Sunday June 28, 2015
June 27 
Day 2
ON CAMPUS
Apply principles and theories from Chapters 4-7 to perform laboratory activities.

Print out the following documents before coming to lab:

Lab 5. DNA Isolation
Lab 6. Polymerase Chain Reaction
Lab 7. Electrophoresis
Lab 8. Biorad PV92
Upload completed Study Questions into Assignments Day 2 in BlackBoard - Due Date July 5, 2015

IMPORTANT: Please go into BlackBoard and review the Biorad PV92 Kit instruction booklet and review the PV92 PowerPoint presentation BEFORE coming to lab.

June 29 - July 5 Review Chapter 9 Review the "Scanning Life's Matrix: Genes, Proteins, and Small Molecules" presentation
July 6 - 12 Review Chapters 10 and 11 DNA Sequencing Assignment
Exam 2 Covers Labs 5-8, Deadline Sunday July 12, 2015
July 13 -19 Review Chapters 12
  1. Review Microarray Manufacturing Technology presentation.
  2. Review "How to Analyze DNA Microarray Data" presentation.
  3. Review the Virochip DNA Microarray presentation.
July 18
Day 3
ON CAMPUS
Review Chapter 14

The following documents are located in BlackBoard Day 3 labs. Print out before coming to lab:
Lab 9. DNA Fingerprinting
Lab 10. Genetically Inherited Disease Testing
Lab 11. In Search of the Cancer Gene Testing
Lab 12. Paternity Testing
Upload completed Study questions into Assignments Day 3 in BlackBoard - Due Date July 26, 2015

July 20 - 26 Review Chapter 13 Review the "DNA Fingerprint: Alu" information prior to lab.
July 27 - Aug 2 Review Chapter 15 Molecular Oncology Assignment
Exam 3 Covers Labs 9-12, Deadline August 2, 2015
July 28- Aug 3  
  1. Review DNA Sequence Assembly presentation answer the questions on the worksheet and submit to assignments in BlackBoard
  2. Review the Sanger Method of Sequencing video
  3. Work on Study Questions for Labs 13 and 14 so they can be graded and returned.
  4. Review objectives for on campus lab, Day 4. Start completing in preparation for Exam 4.
Aug 8
Day 4
ON CAMPUS

  Go to BlackBoard for documents.
Worksheets 1 through 7 - Forensics, Identity Testing, DNA Fingerprinting, Paternity Testing, etc.
Lab 13 Principles of DNA Sequencing
Lab 14 Sequencing the Human Genome
Upload into Assignments Day 4 BlackBoard - Due Date August 12, 2015
Exam 4 Covers Worksheets and Labs 13-14, Deadline August 12, 2015
COMPREHENSIVE FINAL EXAM, August 12 - 16, 2015

 

Last Update: June 29, 2015
Web Author: Terry Kotrla, MS, MT(ASCP)BB
Original publication date: January 2008
Comments: kotrla@austincc.edu