Candle Jar

Any sealable, non-flammable jar large enough to hold petri dishes can be a candle jar. In this experiment, you will spot streak nutrient agar plates with several organisms.  You will then put the plates in a petri dish can, and your instructor will light a tea light and seal the jar with the lit candle inside. The candle flame will consume most of the oxygen in the jar and will produce an elevated level of carbon dioxide. These conditions are ideal for the growth of microaerophilic organisms.